By Richard Twyman
This booklet emphasises the unifying ideas and mechanisms of molecular biology, with widespread use of tables and bins to summarise experimental info and gene and protein features.
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This e-book emphasises the unifying ideas and mechanisms of molecular biology, with common use of tables and bins to summarise experimental information and gene and protein services.
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Extra info for Advanced molecular biology: a concise reference
IRL Press, Oxford. W. and Hunt, T. Introduction. Freeman,NewYork. A. Y. (1997)Targets of checkpointscontrollingmitosis:Lessonsfromlower eukaryotes. Trends Cell Biol. 7 283-288. N. (1997)The cyclin box fold protein recognition in the cell cycle and transcriptional co trol. Trends Biochem. Sci. 22: 482-487. 1948-1949. D. (1993) The cell cycle of E. coli. Annu. Pines, J. andHunter, T. (1995) Cyclin-dependent kinases: An embarrassment of riches? In:Cell Cycle Rev. Microbiol. 4 7 199-230. M. D. Glover),pp.
G. and Errington,J. (1995)Chromosome parTrends Genet. 13 222-226. Nasmyth, K. Annu. Rev. Genet. 2 9 41-67. cell cycle. Trends Genet. 12 405-412. R. B. (1997)'Qing it all together: Epigenetics, genetics, cell cycle and cancer. Science 277 Chapter 3 Chromatin I I Fundamentalconceptsanddefinitions The DNA in eukaryotic chromosomes exists in a complex with an approximately equal mass of protein, forming a highly ordered nucleoprotein substance termed chromatin. The protein component of chromatin consists primarily of histones plus a small amount of other proteins, collectively termed nonhistones.
2 Heterokaryon experiments to investigate regulatoryfactors controlling the cell cycle Fusion Result replicate nuclei Both S X G1 S X G2 M x G,, S or G2 G1 Dresent v x G2 are mitosis S-phase cellcompletesreplication, Gp-phase nucleus waits for S-phase nucleus to complete replication and then both cells enter the M phase Interphase nucleus enters precocious mitosis (regardless of state of chromosome replication) Neither nucleus undergoes replication Both or Conclusion S nucleus contains an S-phase promoting factor G2 nucleuscannotrespond to S-phase activator (a re-replication block), S-phase activator is also an inhibitor of mitosis M nucleus contains an M-phase promoting factor S-phase and M-phase activators 26 Advanced Molecular Biology transplantation studies -interphase nuclei formed spindles when injected into eggs arrested at the metaphase of meiosis I, and cytoplasmfrom these eggs could inducemeiosis in oocytes arrested at G2.
Advanced molecular biology: a concise reference by Richard Twyman