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Daniel L. Purich's Advances in Enzymology and Related Areas of Molecular PDF

By Daniel L. Purich

ISBN-10: 0471349216

ISBN-13: 9780471349211

The Advances in Enzymology and comparable parts of Molecular Biology Series is among the such a lot prestigious within the box, dedicated to the most recent traits in molecular biology and enzymology. every one quantity of the sequence comprises contributions from prime specialists within the box. lower than Dr. Purich's editorship, which started with quantity seventy two, the sequence has accelerated its assurance to incorporate thematic volumes concentrating on particular study components, in addition to non-thematic volumes together with chapters with a extra normal allure.

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Additional info for Advances in Enzymology and Related Areas of Molecular Biology, Mechanism of Enzyme Action

Sample text

Evans and Slotin (63) have since presented evidence that there is, in fact, no fixation of carbon dioxide by pigeon breast muscle. The action of carboxylase in pyruvate oxidation, according to Krebs and Eggleaton’s concept, is to synthesize oxalacetate which is necessary as a hydrogen carrier and as a component of the Krebs cycle. The stimulating action of cocarboxylase in apparently dissimilar reactions, i. were enough alike so that they would be catalyzed by aimilar or identical enzymes, both requiring cocarboxylaae.

With pigeon breast muscle it waa necessary to add a C4 dicarboxylic acid to the malonate-inhibited reaction in order to get oxidation of pyruvate. Apparently breast muscle cannot synthesize C4 dicarboxylic acids under the conditions as can liver. The only malonate-insensitive reaction that had been described in the literature for synthesis of C4 dicarboxylic acids is the fixation reaction studied by Wood and Werkman (41, 42). The fact that oxalacetic acid is an intermediate in the Krebs cycle further added to the attractiveness of the hypothesis that the oxalacetate is formed by the fixation reaction occurring in the dissimilation of pyruvate by pigeon liver.

B. FIXATION OF CARBON DIOXIDE INVOLVJNG A CARBON TO CARBON LINKAQE 1. COOH The reader is referred to this discussion (p. 153) for a more detailed account concerning the above fixation reaction, for despite Krebs' statement, no direct evidence has been obtained as yet with animal tissue which permits the definite conclusion that pyruvic or oxalacetic acid is a component of the fixation reaction. HETEROTROPHIC ASSIMILATION OF CARBON DIOXIDE 39 Apparently carbon dioxide fixation with formation of a carbon to carbon linkage has been demonstrated in only one tissue-liver.

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Advances in Enzymology and Related Areas of Molecular Biology, Mechanism of Enzyme Action by Daniel L. Purich

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