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Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby,'s Affinity Techniques - Enzyme Purification: Part B PDF

By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek

ISBN-10: 0121818977

ISBN-13: 9780121818975

The significantly acclaimed laboratory common, Methods in Enzymology, is likely one of the so much hugely revered guides within the box of biochemistry. due to the fact that 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this day - really a vital booklet for researchers in all fields of existence sciences

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Extra resources for Affinity Techniques - Enzyme Purification: Part B

Sample text

1. Model chromatogram illustrating biospecific affinity chromatography with one-step elution (displacement) using a nonspeciflc displacer. Striped areas represent impurities. [2] GENERAL METHODS AND COUPLING PROCEDURES 17 Concentration "///× ~4q ~//~ J ~/,/', .... J J~ J f// Strong displacer rinsing) Effluent volume FIG. 2. Model chromatogram illustrating fractionation and incomplete purification of specifically adsorbed substances by means of a concentration gradient of a nonspecific eluting agent ( - - - ) .

By including glycerol, may have a beneficial effect when hydrophobic bonding is contributing significantly to the formation of the adsorption complex or when an excess of a hydrophobic spacer substituent is likely to obscure the specific character of the adsorption. Specific Displacement and Gradient Elution Selectivity in purification can be increased by the use of biospecifically mediated desorption as well as adsorption. The desorption can obviously be accomplished by inclusion in the eluent of species competing with the ligand.

The substance A at a certain concentration is continuously introduced into the top of the bed and the effluent is analyzed for detection of the breakthrough of the substance A. The position of the front in such a chromatogram, V'~, depends on the concentration and the affinity (association) constant. If equilibrium is attained under the prevailing chromatographic conditions, adsorption isotherms may be established based on the retention volumes. If we further assume that all adsorption centers in the gel are equally strong and that interaction will occur only with one single group or region in the solute molecule, the following expression for the relative retention S can be deduced: S = (V',- V ~ ) / ( V , - Vo) = (K~v.

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Affinity Techniques - Enzyme Purification: Part B by Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek


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