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An Engineering Introduction to Biotechnology by J. Patrick Fitch PDF

By J. Patrick Fitch

ISBN-10: 0819444979

ISBN-13: 9780819444974

This educational can help technical execs in optics make certain even if their applied sciences have power program within the existence sciences. It is also valuable as a ''prep class'' for extra specified books on biology and biotechnology, filling the distance among basic and high-level approaches.

Contents

- Preface
- easy Biology
- Nucleic Acids because the Blueprint
- Manipulating Nucleic Acids and Proteins
- An built-in technique for organic Discovery
- DNA Sequencing
- Detecting Nucleic Acids
- Protein Structure
- Appendix A: devices and Measures
- Appendix B: Nonscientific Issues
- advised Reading
- Index

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Additional info for An Engineering Introduction to Biotechnology

Sample text

Different forms of a gene, such as tallness and shortness, are known as alleles. When the genes for a trait are the same, that individual is called homozygous for the trait. When the genes differ, the individual is called heterozygous for the trait. Because it is a dominant trait, Mendel’s pea plants that are heterozygous in the height gene will be tall. Pea plants that are homozygous for the shortness gene will be short. Of course, Mendel made his observations in the context of breeding patterns and not chromosomes.

When the strands are complementary (or very close to Manipulating Nucleic Acids and Proteins 47 complementary), the labeled DNA hybridizes to the spot and its label can be detected. Northern blots are a similar procedure used to separate and identify RNA fragments. Western blots are also similar to Southern blots but are applied to proteins. For proteins, the electrophoresis is usually done in polyacrylamide. After being blotted onto a nylon or nitrocellulose membrane, the proteins are detected with labeled antibodies.

In 1877, Robert Koch showed that this spore-forming bacterium caused disease. This was the first demonstration of bacteria causing disease. 16 Chapter 1 a) Grama -Positive si iv Cell C l wall Peptidoglycan Bilipid Bil id cytoplasmic t asmi membrane m an b) Grama -Negative e ive Outer bilipid membrane m an Periplasmic space spac Peptidoglycan Inner bilipid cytoplasmic c opl sm membrane m an Fig. 9. Cartoon comparing the structural differences between (a) Gram-positive and (b) Gram-negative organisms.

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