By Lyn Healy, Ludmila Ruban
This lavishly-illustrated, authoritative atlas explores the complex paintings of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – conceal quite a few themes in pluripotent stem telephone culturing together with mouse and human fibroblasts, human embryonic stem cells and prompted pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in Culture is a entire selection of illustrated strategies complemented through informative and academic captions reading what high quality cells seem like and the way they behave in a variety of environments. Examples of excellent cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and triggered pluripotent stem mobile colonies. This exact and thorough atlas is a useful source for researchers, academics, and scholars who're attracted to or operating with stem phone culturing.
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Additional resources for Atlas of Human Pluripotent Stem Cells in Culture
For both mouse and human fibroblast sources, the feeders generated require a robust testing procedure to establish over what range of passages and cell density the feeders will support the maintenance of undifferentiated PSCs. A number of fibroblast cell lines have also been shown to maintain undifferentiated PSCs, but although referred to as cell lines, these are usually not immortal and as such have a limited lifespan. The quality of the cultures of fibroblasts is assessed morphologically. When passaged, the cells should adhere and should start to spread out.
Some areas in the centre of the colonies are still undifferentiated (black arrows). Outside of the colony is a wide border of totally differentiated cells (white arrows). (b) Three colonies: a Number 1 has a perfect morphology. Number 2 is partially differentiated (black arrows). Number 3 is differentiated and is beginning to form a neuronal rosette (both ×4 magnification) b c Fig. 14 In these images, hESC colonies have grown into the empty spaces created by removing or scraping away colonies. This has proved to be a very useful method during the derivation of cell lines for regrowth of colonies; it can be observed in old culture dishes kept and maintained after mechanical removal of some colonies.
Fernandes AM, Meletti T, Guimarães R, Stelling MP, Marinho PA, Valladão AS, Rehen SK. Worldwide survey of published procedures to culture human embryonic stem cells. Cell Transplant. 2010;19:509–23. Lee JB, Song JM, Lee JE, Park JH, Kim SJ, Kang SM, et al. Available human feeder cells for the maintenance of human embryonic stem cells. Reproduction. 2004;128:727–35. Leonardo TR, Schell JP, Tran HT, Peterson SE. Preparation of mouse embryonic fibroblast feeder cells. In: Loring JF, Peterson SE, 3 Inactivated Mouse and Human Fibroblasts editors.
Atlas of Human Pluripotent Stem Cells in Culture by Lyn Healy, Ludmila Ruban