By Robin Lane Fox
Saint Augustine is among the such a lot influential figures in all of Christianity, but his route to sainthood was once not at all guaranteed. Born in advert 354 to a pagan father and a Christian mom, Augustine spent the 1st thirty years of his existence suffering to appreciate the character of God and his global. He realized approximately Christianity as a toddler yet was once by no means baptized, identifying as a substitute to immerse himself within the learn of rhetoric, Manicheanism, after which Neoplatonism—all the whereas indulging in a lifetime of lust and greed.
In Augustine, the acclaimed historian Robin Lane Fox re-creates Augustine's formative years with unprecedented perception, exhibiting how Augustine's quest for wisdom and religion eventually introduced him to Christianity and a lifetime of celibacy. Augustine's Confessions, a bright description of his trip towards conversion and baptism, nonetheless serves as a version of spirituality for Christians round the world.
Magisterial and wonderfully written, Augustine may be the definitive biography of this significant determine for many years to return.
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Extra resources for Augustine: Conversions to Confessions
FACS analysis is performed on a Beckon Dickinson FACS Caliber machine with a 488-nm laser. 8. Mean EGFP is the average emission in relative fluorescence units (RFU) of approx 10,000 cells that reside within a gated value set at a threshold above background fluorescence. 5. Measuring Exon IIIb Inclusion Using pGIIIb Plasmids The plasmid pEGFPN1 (Clontech, Palo Alto, CA), which can drive EGFP expression in both DT3 and AT3 cells, was used to construct the pG family of alternative splicing reporter vectors.
Several factors have been implicated in mediating IIIb silencing; the polypyrimidine tract binding protein (PTB) binds the ISS elements that flank IIIb and silences this exon (11). HnRNP A1 has also been implicated in IIIb silencing. Artificial recruitment of hnRNP A1 protein to IIIb leads to decreased IIIb inclusion (18). IIIb inclusion is likely mediated by an epithelial specific factor, which we posit interacts with and stabilizes a secondary structure predicted to be formed by ISAR-IAS2 base pairing (7,9).
Change the media every 3 d during this incubation. Trypsinize the flask, then replate into a T75 flask in the same selective media (see Note 14). After sufficient numbers of blasticidin/hygromycin resistant cells have been produced, the cells are ready for flow cytometry analysis. 4. 5. 6. 3. Fluorescent Microscopy 1. Cells are plated at 3 × 105/well in a six-well plate, where each well contains a sterile glass coverslip. 2. The following day, remove the media and wash the cells once with 2 mL PBS.
Augustine: Conversions to Confessions by Robin Lane Fox