By Byron Rubin, Edward A. Dennis (Eds.)
The severely acclaimed laboratory usual for greater than 40 years, Methods in Enzymology is among the so much hugely revered courses within the box of biochemistry. seeing that 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. greater than 270 volumes were released (all of them nonetheless in print) and masses of the fabric is appropriate even today--truly an important book for researchers in all fields of lifestyles sciences
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F. Koetzle, G. J. B. Williams, E. F. Meyer, M. D. Brice, J. R. Rodgers, O. Kennard, T. Shimanouchi, and M. Tasumi, J. Mol. Biol. 112, 535 (1977). 2 E. Abola, F. C. Bernstein, S. H. Bryant, T. F. Koetzle, and J. Weng, in "Crystallographic Databases--Information Content, Software Systems, Scientific Applications" (F. H. Allen, G. Bergerhoff, and R. ), p. 107. Data Commission of the International Union of Crystallography, Bonn/Cambridge/Chester, 1987. 3 N. Pattabiraman, K. Namboodiri, A. Lowrey, and B.
Cygler, unpublished data, 1997). These lipases have molecular masses close to 30 kDa. Their lids are formed by a single loop, which is approximately 15 residues long. In those structures in which the lid was well ordered in the crystal, its middle segment was folded into an ot helix. Residues Ser-82 to Pro-96 constitute the lid in R. miehei lipase (homolog of H. lanuginosa), situated on the N-terminal side of the active site serine (Fig. 4). Interestingly, the location of the lid corresponds topologically to strand/34 (and the following helix), which is missing in these lipases as compared to other a//3-hydrolase fold enzymes.
Browsing one of the existing classifications of protein structures will probably give essentially the same information. Initial Alignment of Three-Dimensional Structures For well-conserved sequences it is easy to set up a structural alignment by hand, using a standard modeling package with options for superposition of macromolecules based on RMS (root-mean-square) deviations. , by an initial sequence alignment, and superimposing those motifs. However, for less well-conserved sequences it is normally easier and less biased to use an automatic approach.
Lipases, Part A: Biotechnology by Byron Rubin, Edward A. Dennis (Eds.)