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New PDF release: Membrane structural biology: with biochemical and

By Mary Luckey

ISBN-10: 0521856558

ISBN-13: 9780521856553

Membrane Structural Biology brings jointly a physicochemical research of the membrane with the most recent structural biology on membrane lipids and proteins to provide an exhilarating portrayal of biomembranes. Written with awesome readability, this article seems to be at a time while membranes have moved again into the medical highlight and may offer a distinct starting place for complicated scholars and dealing scientists. The constitution, functionality, and biogenesis of membrane lipids and proteins are tested, bioinformatics and computational methods to membrane elements are brought, and the high-resolution buildings which are giving new insights into the important roles membranes play are mentioned. the numerous correlations among membrane learn and human healthiness are mentioned and key issues for destiny paintings during this sector are pointed out. Membrane structural biology is poised to respond to many uncomplicated and utilized questions and this state of the art textual content will supply an exceptional grounding for all these operating during this box.

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Extra info for Membrane structural biology: with biochemical and biophysical foundations

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Wagner, Introduction to Biological Membranes, 2nd ed. New York: Wiley, 1988, p. 53. Bilayer-forming lipids such as PCs that favor lamellar aggregates are roughly cylindrical in shape, with similar cross-sectional areas for the heaetgroup and the acyl chains, This can be described by a shape par'ameter, S, calculated as lipid volume/(cross-sectional area of polar headgroup x lipid length). FN a fairly cylindrical lipid, S equals ~J. A lipid that is conical (S > I) or wedge shaped (S < 1) introduces curvature, leading to the fonnation of non lamellar phases.

B, Biomembranes, Springer-Verlag, 1989, p. 53. 11") 1989. Reprinted with permission from E. Oldfield. tained by organisms living under extreme conditions (as discussed above). Another important source of fluidity is the movement of whole molecules within the two-dimensional matrix of the bilayer Diffusion of Bilayer Lipids Lipid molecules move within the bilayer in three different modes: mtational, lateral, and transverse. Rotational diffusion, the spinning of a single molecule around its axis, affects a lipid's interactions with its nearest neighbors but does not alter its position.

It requires excitation with plane-polarized light and probe on observation through analyzing polarizers to resolve the flulipids orescence intensity into parallel and perpendicular compoView surface with nents. Fluorescence recovery after photobleaching (FRAP) Auorescence uses fluorescence microscopy to follow fluorescence intensity microscope over time after a laser beam destroys the fluorophores in a small observation area. The rate of recovery of fluorescence is a measure of the rate of diffusion of unbleached molecules into the bleached area.

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Membrane structural biology: with biochemical and biophysical foundations by Mary Luckey

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